Journal: Polish Journal of Environmental Studies
Article Title: Assessing the Biocontrol Potential of Some Isolated Bacteriophages Against Salmonella spp. in Food Preservation: A Preliminary Study
doi: 10.15244/pjoes/188905
Figure Lengend Snippet: Fig. 2. Pulsed Field Gel Electrophoresis (PFGE) Genome Length Determination. a) PFGE for 13 phages out of 28 phages appear as single bands on PFGE which could be used to assess nucleic acid size of phages (1, 2, 3, 4, 8, 10, 11, 12, 13, 14, 15, 17) by comparing it to a ladder. PFGE was done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA), the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, Running Time = 9 hours, V= 6 V/cm, Temp = 4°C, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 6.0 software (BioRad); b) BioRad CHEF DNA Lambda Ladder #1703707.
Article Snippet: PFGE was done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA), the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, Running Time = 9 hours, V= 6 V/cm, Temp = 4°C, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel DocTM XR+ UV Trans illumination and ImageLab 6.0 software (BioRad); b) BioRad CHEF DNA Lambda Ladder #1703707.
Techniques: Pulsed-Field Gel, Electrophoresis, Agarose Gel Electrophoresis, Staining, Software